Name:
hs0000506
Species:
Homo sapiens
Cell Line:
MCF7
Restriction Enzyme:
HinfI
Remark:
control: nearby locus; treatment: ɑ-amanitin, 1ɑ,25(OH)2D3; semi-quantitative 3C; VDR silencing to chromatin looping;at time periods 40 –50 and 70 –80 min
Primer amount:
NA
Test repetition:
NA
Reliability Level:
II
| Anchor | |||
|---|---|---|---|
| Locus1 | Fragment location | Primer sequence | Strand |
CYP24,TSS,segment VDRE1/2 |
20:52790409-52790627 | GACCATGCATTTATGGAG | + |
| Target | |||
| Locus2 | Fragment location | Primer sequence | Strand |
|
CYP24,segment VDRE4 |
20:52794250-52794572 | GAGACAGGAGTTTGCCAT | - |
Matilainen, J. M., et al. (2010). "The number of vitamin D receptor binding sites defines the different vitamin D responsiveness of the CYP24 gene in malignant and normal mammary cells." Journal of Biological Chemistry 285(31): 24174-24183. PMID: 20460683
| ©BIG 2015, Beijing Institute of Genomics, Chinese Academy of Sciences
NO.1 Beichen West Road, Chaoyang District, Beijing 100101, China.
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