Detail Information of hs0000315
1.Basic Information

Name: hs0000315
Species: Homo sapiens
Cell Line: adult splenic erythroblasts
Restriction Enzyme: HindIII

2.Experiment Infromation

Remark: βYAC transgene;the expressing β-globin gene is in closer proximity to the LCR in comparison with the non-expressing γ-globin gene;The small HS3 core deletion (0.23 kb), but not the large HS3 deletion (2.3 kb), disrupted the spatial interactions among all the HS sites of the LCR, the β-globin gene and 3'HS1
Primer amount: NA
Test repetition: NA
Reliability Level: III

3.Locus Information

Anchor
Locus1 Fragment location Primer sequence Strand
HBB,3'HS1 NA NA NA
Target
Locus2 Fragment location Primer sequence Strand
HBB,LCR HS2 NA NA NA

4.Reference

Fang, X., et al. (2007). "Cooperativeness of the higher chromatin structure of the beta-globin locus revealed by the deletion mutations of DNase I hypersensitive site 3 of the LCR." J Mol Biol 365(1): 31-37.   PMID: 17056066

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