Name:
hs0000304
Species:
Homo sapiens
Cell Line:
adult splenic erythroblasts
Restriction Enzyme:
HindIII
Remark:
βYAC transgene;the expressing β-globin gene is in closer proximity to the LCR in comparison with the non-expressing γ-globin gene;The small HS3 core deletion (0.23 kb), but not the large HS3 deletion (2.3 kb), disrupted the spatial interactions among all the HS sites of the LCR, the β-globin gene and 3'HS1
Primer amount:
NA
Test repetition:
NA
Reliability Level:
III
Anchor | |||
---|---|---|---|
Locus1 | Fragment location | Primer sequence | Strand |
HBB | NA | NA | NA |
Target | |||
Locus2 | Fragment location | Primer sequence | Strand |
HBB,LCR HS7 | NA | NA | NA |
Fang, X., et al. (2007). "Cooperativeness of the higher chromatin structure of the beta-globin locus revealed by the deletion mutations of DNase I hypersensitive site 3 of the LCR." J Mol Biol 365(1): 31-37. PMID: 17056066
©BIG 2015, Beijing Institute of Genomics, Chinese Academy of Sciences
NO.1 Beichen West Road, Chaoyang District, Beijing 100101, China.
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